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Hydrophobic phase with alternative selectivity compared to classical C₁₈ modifications. The separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. This provides better retention of aromatic and unsaturated substances. This stationary HPLC phase is based on core-shell technology silica gel particles and shows excellent performance under highly aqueous conditions. The special phase modification of NUCLEOSHELL^® Biphenyl with iso-butyl sidechains leads to low bleeding characteristics even at very acidic pH values compared to competitor columns. Due to these iso-butyl sidechains and multi-endcapping procedures no phase collapse occurs and stability in 100 % aqueous mobile phase is ensured.