Angptl1 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS5611-50, BS5611-100

Sizes: 50µl, 100µl

Swiss-Prot: O95841

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Angptl1 (angiopoietin-like 1), also known as angioarrestin, ARP1, ANGPT3 or previously known as angiopoietin 3 (ANG3), is a member of the angiopoietin-like family. It is highly expressed in adult tissues, particularly adrenal gland, thyroid, placenta and small intestine. Angptl1 exists as a disulfide-linked dimer and shares 45.1% identity with Ang-1 and 59% identity with Angptl2. Angptl1 consists of an N-terminus with a coiled-coil domain, potential glycosylation sites and a C-terminus with a fibrinogen-like domain. It is a secreted protein but does not function as a growth factor in endothelial cells. Angptl1 plays a distinct role in the regulation of angiogenesis; inhibiting proliferation, migration, tube formation and endothelial cell adhesion. To exert this inhibitory activity, Angptl1 is speculated to interact with a receptor on endothelial cells. In a wide variety of tumor tissues, Angptl1 expression is down-regulated suggesting that a major function of this protein involves its antiangiogenic properties.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Angptl1 polyclonal antibody detects endogenous levels of Angptl1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Angptl1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat spleen tissue lysate, Immunohistochemistry (IHC) analyzes of Angptl1 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing secreted staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 56 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Angiopoietin-related protein 1; Angiopoietin-3; ANG-3; Angiopoietin-like protein 1; ANGPTL1; ANG3; ANGPT3; ARP1; PSEC0154; UNQ162/PRO188

Immunogen: Synthetic peptide, corresponding to amino acids 58-103 of Human Angptl1.

Conjugate: Unconjugated

Modification: Unmodified