Phospho-FAK-Y576/577 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79395-50, BS79395-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q05397

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat and Raji cells, using Phospho-FAK-Y576/577 antibody at 1:1000 dilution. Jurkat cells were treated by CD-3 for 2 minutes after serum-starvation overnight or treated by PMA/TPA for 10 minutes. Raji cells were treated by PMA/TPA for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 130kDa

Specificity: Phosphorylated

Alternative Name: FADK; FAK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; PTK2

Immunogen: A synthetic phosphorylated peptide around Y576 & Y577 of human PTK2(NP_722560.1).

Conjugate: Unconjugated

Modification: Phosphorylated