GSDMD-NT Polyclonal Antibody

Catalogue Numbers: BS67358-50, BS67358-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P57764

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/2000)

Background: Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis. Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18. Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GSDMD-NT protein

Extra Notes: Western blot analysis of GSDMD-NT expression in THP1 (A) whole cell lysates.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DFNA5L; GSDMDC1; Gasdermin-D; Gasdermin domain-containing protein 1

Immunogen: Recombinant fusion protein of human GSDMD-NT. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified