Centri-Spin 20 spin columns are used for: 

• electroporation nick translation binding applications
• FISH transfection probe labeling
• gene products signal transduction differential display
• antibody labeling transformation studies
• protein labeling receptor analysis
• solid phase array desalting.

Centri-Spin 20 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)

The Centri-Spin 20 gel will provide excellent recovery of DNA fragments with sizes greater than 20 base pairs or 20-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 25 kD can also be efficiently separated / desalted from unwanted low-molecular-weight impurities.

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. 1 Each Centri-Spin 20 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket Centrifuge to remove the intersititial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.

CENTRI-SPIN 20 COLUMNS CAN ALSO BE USED FOR THE FOLLOWING APPLICATIONS:

• Removal of free and labeled dNTP's from DNA/RNA as in:

1. nick translation
2. end-labeling reactions
3. PCR reactions

• Improving Electroporation Transformation Efficiencies
• Removal of hexamers and octamers from primer-walking and random primer labeling
• Desalting, removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions
• Desalting / purification / buffer exchange of peptides or proteins > 25 kD.

These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

ADVANTAGES OF CENTRI-SPIN 20:

• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient 20–50 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format

BENEFITS OF CENTRI-SPIN 20:

• Reliable and consistent
• Time-saving and convenient
• Enhanced stability
• Extended shelf-life
• No special equipment required
• Extremely versatile