Rat Ang-Ⅱ (Angiotensin Ⅱ) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-R1430-24, E-EL-R1430-48, E-EL-R1430-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: Ang-Ⅱ

UNIProt ID: P01015(Angiotensinogen,25 – 32)

Target Synonyms: Ang-II; Angiotensin-2;

Research Areas: Cancer, Cardiovascular, Metabolism, Signal transduction

Target Species: Rat

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Rat Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Rat Ang-Ⅱ in samples. No significant cross-reactivity or interference between Rat Ang-Ⅱ and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag