Mcl-1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9891M-50, BS9891M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q07820

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Background: B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MCL1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 29, 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MCL1 Rabbit mAb at 1:1000 dilution
Lane 1: Raji whole cell lysate
Lane 2: The heart tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody.Counter stained with hematoxylin., ICC staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT; MCL1; BCL2L3

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified