MelanA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90846-50, BS90846-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16655(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
IHC:1:50-1:200

Background: Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MelanA polyclonal antibody detects endogenous levels of MelanA protein.

Bioworld Molecular Weight: 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1,000 dilution.
Positive control:
Lane 1: Melanoma
Lane 2: B16-F1, Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody

Alternative Name: MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA, Antigen SK29-AA, MAR1, MART 1, MART-1, MART1, Melan A, Melan A protein, Melanoma antigen recognized by T cells 1, Melanoma antigen recognized by T-cells 1, MLAN A, MLANA, OTTHUMP00000021036, OTTHUMP00000021037, OTTHUMP00000021038, Protein Melan-A,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified